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HyperScript III RT SuperMix for qPCR with gDNA Remover   

貨    　號：R202

保存條件：- 20 ℃長(chang)期(qi)儲存(cun)

產品介紹：

本品為(wei)針(zhen)對RT-qPCR優(you)化(hua)的(de)(de)(de)逆轉(zhuan)(zhuan)錄試劑盒(he)，包含(han)gDNA去除和逆轉(zhuan)(zhuan)錄兩管mix，使(shi)用(yong)時只(zhi)需(xu)加(jia)入RNA模板并補(bu)足水即可。高濃度的(de)(de)(de)8 x gDNA remover不僅預(yu)留充足的(de)(de)(de)RNA加(jia)樣(yang)體積（以應(ying)對低(di)濃度的(de)(de)(de)RNA），同時可以快(kuai)速去除RNA樣(yang)本中殘留的(de)(de)(de)基(ji)因(yin)組DNA，使(shi)得(de)qPCR的(de)(de)(de)引物(wu)無(wu)需(xu)跨內(nei)含(han)子設計(ji)。試劑盒(he)所(suo)使(shi)用(yong)的(de)(de)(de)逆轉(zhuan)(zhuan)錄酶(mei)是基(ji)于M-MLV定向進化(hua)而來的(de)(de)(de)新一(yi)代逆轉(zhuan)(zhuan)錄酶(mei)，具有更低(di)的(de)(de)(de)RNase H活性(xing)、更高的(de)(de)(de)熱穩定性(xing)和cDNA合成效(xiao)率，搭配(pei)針(zhen)對qPCR優(you)化(hua)的(de)(de)(de)逆轉(zhuan)(zhuan)錄引物(wu)配(pei)比，可以獲得(de)在RNA各個位置逆轉(zhuan)(zhuan)錄效(xiao)率均一(yi)的(de)(de)(de)cDNA。

產(chan)品組成：

溫馨提示：

·5 × RT SuperMix包(bao)含(han)逆轉(zhuan)錄(lu)所需的全部組分，包(bao)含(han)Randomprimers/Oligo (dT)20VN primer mix，因此不適用于基(ji)因特異性   

   引物進行(xing)逆(ni)轉(zhuan)錄，如有該需求推薦使用NovaBio R201。

·8 × gDNA remover、5 × RT SuperMix含有高(gao)濃(nong)度的甘(gan)油，使(shi)用前請短暫離心，避免槍(qiang)頭插入管底(di)吸取試劑。

·該試(shi)劑盒的cDNA產(chan)物僅(jin)適用于qPCR實驗，若需要獲得全長(chang)的cDNA，推薦使用NovaBio R201。

·為保證實驗(yan)成功率和準確性，請勿使用過度(du)降解的RNA。

·配(pei)置反應(ying)液時，請(qing)使用RNase-free槍(qiang)頭(tou)、Microtube等。

產品(pin)使用：

1.gDNA去除(chu)體系配置及反應程序(xu)

混勻后(hou)42℃ 2min。

注：如果加(jia)入的為mRNA：10 pg - 500 ng。

2.配(pei)置逆轉錄反應體系

請配(pei)置如下反應液：

3.逆轉錄(lu)反應程序(xu)

注：1. HyperScript^TM III 在37-60℃均有較強活性，37℃即可滿足(zu)絕大多數實驗；若目標RNA區(qu)域有高(gao)GC部分，可提(ti)高(gao)逆轉錄溫度(du)至50℃。

       2. 85℃ 5s 可以失(shi)活逆轉錄酶和gDNA remover，請(qing)勿省略該步驟。

逆轉錄產物可直接用于(yu)下游實驗，也可置于(yu)4℃短暫放(fang)置，3-6個(ge)月內-20℃保(bao)存，長期存放(fang)請置于(yu)-80℃冰箱，小份分(fen)裝避免反復凍融(rong)。

本品僅供科研使用，不能用于人和動物醫療診斷

使用本(ben)品發表文(wen)章：

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